Highlights

1)This product is a sterile solution;

2)Derived from genetically recombined yeast strains;

3)Does not contain DNase and RNase;

4)Active and stable in urea and SDS;

5)Activity unit definition: at 37°C and pH 7.5, the amount of Proteinase K required to release 1 µM tyrosine per minute is defined as 1 activity unit;

6)Inactivation method: Incubate at 95°C for 10 minutes or at 98°C for 5 minutes to completely inactivate.

Introduction

     Proteinase K is a subtilisin-like protease with serine as the active center, with a relative molecular weight of about 29.3 kDa. It has a wide range of substrate specificity, and preferentially decomposes the C-terminus adjacent to hydrophobic amino acids, sulfur-containing amino acids, and aromatic amino acids. ester and peptide bonds. It has high activity in the buffer solution for extracting DNA and RNA, and is mainly used for protein digestion during DNA and RNA extraction.

Composition

Related products