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FlaPure Animal Tissue Total RNA Extraction Kit(RE715)

FlaPure Animal Tissue Total RNA Extraction Kit(RE715)

1) Unique genomic DNA filter column without DNase treatment.
2) No toxic reagents such as DTT and β-mercaptoethanol are required.

＄ 177.00
177.00
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Product Description

1) Unique genomic DNA filter column without DNase treatment.
2) No toxic reagents such as DTT and β-mercaptoethanol are required.


Product Highlights


      1) Easy to operate: complete the extraction of total RNA from several samples within 30 minutes;

2) Efficient removal of genomic DNA: unique genomic DNA filter column without DNase treatment;

3) Safe and low toxicity: no toxic reagents such as DTT and β-mercaptoethanol;

4) High RNA purity: The extracted RNA has no residual impurities and is suitable for downstream experiments that require high purity and integrity




Introduction


      This kit can rapidly extract total RNA from ≤20 mg animal soft tissue (liver, spleen, kidney, brain, etc.), ≤1×107 cultured cells. The kit is based on silica gel column purification technology, no need to use DTT and β-mercaptoethanol during the extraction process, and the entire extraction process can be completed within 30 minutes. The kit combines DNA filtration technology, which can efficiently filter and remove genomic DNA. The extracted total RNA has high purity and is free from protein and other impurities. It can be used for RT-PCR, Northern Blot, Poly A purification, RNase protection analysis and in vitro translation, etc. experiment.



Composition


Contents

Amount(50 rxns)

Buffer FRL

30 mL

Buffer FRW

40 mL

Buffer FRW1

12 mL

Proteinase K

500 µL

RNase-Free ddH2O

15 mL

RNase-Free FlaPure RNA Columns

50

RNase-Free FlaPure gDNA Remove Columns

50

Collection Tubes(2.0 mL)

100



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Introduction

Reverse Transcription

UnionScript First-strand cDNA Synthesis Mix for qPCR(with dsDNase)

SR511

100 rxns

There are DNase and RNase inhibitors in the master mix. Genome removal and reverse transcription can be completed in one step. The reaction temperature was 50°C.

qPCR

GS AntiQ qPCR SYBR Green Master Mix

SQ412

5×1.0 mL

Using antibody-modified new hot-start GS AntiQ DNA polymerase and carefully optimized Buffer. It is  equipped with two ROX Reference Dye (High/Low) according to different instrument models.



Q1：Why do the columns get clogged?

A1：

1) Too much sample. The lysate is viscous. Reduce the amount of sample or increase the amount of lysate. Too much sample will reduce yield and purity.

2) Insufficient centrifugation of lysate. The tissue lysate needs to be centrifuged at high speed to remove impurities, which will cause blockage of the column.

3) The centrifugation temperature is too low. The operation of this product is carried out at room temperature.

 

Q2：Why do gDNA filter cartridges clog during RNA extraction?

A2：

1) Increase the number of centrifugations, time or revolutions;

2) The filtrate can be sucked out and added to an appropriate amount of lysate to homogenize and then transferred to a new gDNA filter column.


Manual: Click to download

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