Q1：Why do the columns get clogged?
A1：
1) Too much sample.Take appropriate samples according to the requirements recommended in the instructions;
2) For starch rich samples or mature leaves,please increase Buffer GSL to 700 μ L;
3) The centrifugation temperature is too low. The operation of this product is carried out at room temperature.
Q2：Why does RNA degrade?
A2：
1）Too much sample will affect the cleavage capacity of the lysate, and RNase is not sufficiently inhibited, leading to RNA degradation.It is recommended to refer to the recommended sampling amount in the instructions.If the sample mass is to be increased, the amount of each solution in subsequent experiments should be increased in an equal proportion.For tissues with high endogenous RNase content, the sample should be reduced and the amount of lysate should be appropriately increased;
2) Improper storage of samples. Repeated thawing can cause RNA degradation. Try to use fresh samples or samples that are thawed no more than twice;
3) Introduce RNase during operation. Please use RNase-Free tools, reagents, and consumables;
4) Degradation occurs during electrophoresis. Using RNase-Free Loading Buffer, Agarose, and Electrophoresis Buffer.
