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Lysis Buffer for Direct PCR(Mouse Tissue)(SL312-100)

Lysis Buffer for Direct PCR(Mouse Tissue)(SL312-100)

A lysis buffer for mouse tissue.

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Product Description

A lysis buffer for mouse tissue.


Highlights


1) Ease of use: no genomic DNA extraction required.

2) Wide application: suitable for direct expansion of various mouse tissues.





Introduction


   This product can directly use mouse tail, ears, toes and other tissues for PCR amplification after simple lysis treatment with Lysis Buffer and Proteinase k, without overnight digestion, phenol-chloroform extraction or column purification. Experiment time.



Composition


Contents

Amount

Lysis Buffer

2×20 mL




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Q1Why there is no ORF in the final result?

A1:

1) Excessive cleavage products. Select the most suitable template dosage, generally not more than 1/10 of the system;

2) The sampling volume is too large. Dilute the cleavage product by 10 times and then amplify, or reduce the sampling amount and re-lyse;

3) The tissue sample is not fresh. Fresh tissue samples are recommended;

4) Poor primer quality. Use genomic DNA for amplification to verify primer quality and optimize primer design.


Q2Why does the final result show non-specific amplification?

A2:

1) The annealing temperature is too low and the number of cycles is too high. Increase the annealing temperature and reduce the number of cycles;

2) The template concentration is too high. Reduce the amount of template or dilute the template by 10 times for amplification;

3) Poor primer specificity. Optimize primer design.



Manual: Click to download

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