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FlaPure Plant Total RNA Extraction Plus Kit(RE713)

FlaPure Plant Total RNA Extraction Plus Kit(RE713)

This product is suitable for plant tissue rich in polysaccharides, polyphenols, or starch.

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Product Description

This product is suitable for plant tissue rich in polysaccharides, polyphenols, or starch.

Product Highlights

      1) Easy operation: complete the total RNA extraction of several samples within 30~40 min.

2) Efficient removal of genomic DNA: Unique genomic DNA filter column and DNase I digestion to efficiently remove genomic DNA.

3) Safe and low toxicity: No toxic reagents such as phenol and chloroform are required.

4) High RNA purity: The extracted RNA has no impurities, which is suitable for downstream experiments that require high purity and integrity.


      This kit is suitable for rapid extraction of total RNA from plant tissues rich in polysaccharides, polyphenols, or starch. The kit is based on silica gel column purification technology, no need to use phenol and chloroform  in the extraction process, and the entire extraction process can be completed within 30~40 minutes. Combined with DNA filtration technology and DNase, the kit can efficiently filter and remove genomic DNA. The total RNA extracted is of high purity, free of protein and other impurities, and can be used for RT-PCR, Northern Blot, Poly A purification, RNase protection analysis and in vitro translation, etc. experiment.



Amount(50 rxns)

Buffer GSL

30 mL

Buffer GRW1

40 mL

Buffer GRW2

12 mL

RNase-Free ddH2O

15 mL

RNase-Free DNase I

100 µL

DNase Buffer1.5 mL

RNase-Free FlaPure RNA Columns


RNase-Free FlaPure gDNA Remove Columns


Collection Tubes(2.0 mL)


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Using antibody-modified new hot-start GS AntiQ DNA polymerase and carefully optimized Buffer. It is  equipped with two ROX Reference Dye (High/Low) according to different instrument models.

Q1Why do the columns get clogged?


1) Too much sample.Take appropriate samples according to the requirements recommended in the instructions;

2) For starch rich samples or mature leaves,please increase Buffer GSL to 700 μ L;

3) The centrifugation temperature is too low. The operation of this product is carried out at room temperature.

Q2Why does RNA degrade?


1)Too much sample will affect the cleavage capacity of the lysate, and RNase is not sufficiently inhibited, leading to RNA degradation.It is recommended to refer to the recommended sampling amount in the instructions.If the sample mass is to be increased, the amount of each solution in subsequent experiments should be increased in an equal proportion.For tissues with high endogenous RNase content, the sample should be reduced and the amount of lysate should be appropriately increased;

2) Improper storage of samples. Repeated thawing can cause RNA degradation. Try to use fresh samples or samples that are thawed no more than twice;

3) Introduce RNase during operation. Please use RNase-Free tools, reagents, and consumables;

4) Degradation occurs during electrophoresis. Using RNase-Free Loading Buffer, Agarose, and Electrophoresis Buffer.

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