Unizol Total RNA Extraction Reagent (RE703)

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Unizol Total RNA Extraction Reagent (RE703)

Unizol Total RNA Extraction Regent is a broad-spectrum total RNA extraction reagent based on guanidine isothiocyanate/phenol.

List price：＄ 518.00

Price：＄ 518.00

Cat. No.：

RE703

Amount：

100 mL

Quantity：

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Product Description

Unizol Total RNA Extraction Regent is a broad-spectrum total RNA extraction reagent based on guanidine isothiocyanate/phenol.

Highlights

1) Wide range of applications.

2) Simple and fast operation, the whole process can be completed within 1 h.

3) The operation is visible, and the solution is pink, which is easy to separate the aqueous phase and the organic phase.

Introduction

Unizol Total RNA Extraction Regent is a broad-spectrum total RNA extraction reagent based on guanidine isothiocyanate/phenol. It has strong lysis ability, can lyse cells and tissue samples in a short time, and effectively inhibit RNase activity to ensure RNA integrity.

This product is suitable for plant tissues (such as seedlings, young leaves, etc.), cultured cells, animal tissues and microorganisms with less secondary metabolism. The extraction process can be completed within 1 h. The extracted total RNA has high purity and integrity, and removes impurities such as protein and genomic DNA to the greatest extent. It can be directly used in RT-PCR, Northern Blot, in vitro translation and mRNA purification experiments.

Composition

Contents

Amount

Unizol Total RNA Extraction Reagent

100 mL

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Q1：Why does RNA degrade?

A1：

1) Improper sample handling or storage. Try to use fresh samples. Immediately after sampling, freeze them in liquid nitrogen and store them in a -80°C refrigerator. Use them as soon as possible.

2) The sample was used in excess. Please refer to the manual for the recommended sampling volume.

3) RNase is contained in the environment, reagents or consumables. Use RNase-free reagents and consumables, it is recommended to operate in a fume hood.

Q2：Why RNA yields are low?

A2：

1) Insufficient sample grinding or lysis. It is recommended to refer to the instruction manual to fully grind and shake the sample to fully lyse the sample.

2) The sample size is small. It is recommended to increase the sample size appropriately.

3) The RNA precipitate was not completely dissolved. The RNA should not be over-dried, if necessary, gently pipette or incubate at 55~60°C for 5~10 min.

Manual: Click to download

[1] Li N, Wang Z, Yang F et al. MiR-29b Downregulation by p53/Sp1 Complex Plays a Critical Role in Bleb Scar Formation After Glaucoma Filtration Surgery. Translational Vision Science Technology. 2023,12(12):5.

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