Welcome to Genesand  Biotech Co.,Ltd
0086010-400-688-7890
   
PRODUCTS
Triumfi Plant Direct PCR Kit (SD311)

Triumfi Plant Direct PCR Kit (SD311)

This product can be used for direct amplification of tissue samples from plant roots, stems, leaves, etc. It is easy to use and greatly shortens theexperimental time. 

1299.00
1299.00
Amount:
Cat. No.:
Quantity:
Buy now
Add to cart
  
Product Description

This product can be used for direct amplification of tissue samples from plant roots, stems, leaves, etc. It is easy to use and greatly shortens theexperimental time. 




Highlights


1. Easy to operate: no need to extract genomic DNA.

2. Wide application: suitable for direct amplification of various plant tissues.




Introduction

   

  This product is a rapid identification kit suitable for various types of samples, including crude DNA extraction and PCR amplification systems. This product can be used for direct amplification of tissue samples from plant roots, stems, leaves, etc. The tissue can be simply lysed with Lysis Buffer and then subjected to PCR amplification without phenol-chloroform extraction or column purification. It is easy to use and greatly shortens the experimental time. The product is suitable for the amplification of target fragments within 3kb.

      The 2×Plant Direct PCR Mix provided by this product contains genetically engineered DNA polymerase, Mg2+, dNTPs and an optimized buffer system, which has extremely high amplification efficiency and inhibitor tolerance. Add template and primers, and add water to 1× to carry out PCR reaction. The PCR product amplified by this product has a protruding "A" base at the 3' end, which can be directly used for TA cloning after purification (Cat. No. : TC601).




Composition



Contents

Amount

2× Plant Direct PCR Mix

5×1.0 mL

Direct Lysis Buffer

60 mL

Dilution Buffer

20 mL






Related products


Type

Composition

Cat. No.

Amount

Introduction

Nucleic Acid Electrophoresis

Agarose

AG801

100 g

High purity and clear background

Nucleic Acid Electrophoresis

GS-GelRed(10,000×)

GL802

500 µL

Safe and non-toxic; High sensitivity

Nucleic Acid Electrophoresis

100 bp DNA Ladder

SM813

100 rxns

Unique technology makes Marker less susceptible to degradation; The size of the strip is very precise, and the strip type is clear.




Q1: Why does the end result have no purposeful bands?

A1:

1) Excessive cleavage products. Select the most suitable template dosage, generally not more than 1/10 of the system.

2) The sampling volume is too large. Dilute the cleavage product by 10 times and then amplify, or reduce the sampling amount and re-lyse.

3) The tissue sample is not fresh. Fresh tissue samples are recommended.

4) Poor primer quality. Use genomic DNA for amplification to verify primer quality and optimize primer design.

Q2: Why does non-specific amplification occur?

A2:

1) The annealing temperature is too low and the number of cycles is too high. Increase the annealing temperature and reduce the number of cycles.

2) The template concentration is too high. Reduce the amount of template or dilute the template by 10 times for amplification.

3) Poor primer specificity. Optimize primer design.


CONTACT US
   
Telephone Number
0086010-400-688-7890