To help life science research,
let more research results benefit mankind
To help life science research,
let more research results benefit mankind
1)Suitable for direct expansion of various mouse tissues.
2)No genomic DNA extraction required.
Highlights
1) Ease of use: no genomic DNA extraction required.
2) Wide application: suitable for direct expansion of various mouse tissues.
Introduction
This product is a kit specially designed for rapid identification of mouse genotype, including crude DNA extraction and PCR amplification system. This product can directly use mouse tail, ears, toes and other tissues for PCR amplification after simple lysis treatment with Lysis Buffer and Proteinase k, without overnight digestion, phenol-chloroform extraction or column purification. Experiment time. The product is suitable for the amplification of target fragments within 2 kb and multiple PCR reactions within 3 pairs of primers.
The 2×Mouse Tissue Direct PCR Mix provided by this product contains genetically engineered DNA polymerase, Mg2+, dNTPs and an optimized buffer system, which has extremely high amplification efficiency and inhibitor tolerance. The PCR reaction can be carried out after adding template and primers, and replenishing water to 1×. The PCR product amplified by this product has a protruding "A" base at the 3' end, which can be directly used for TA cloning after purification (Cat. No.: TC601).
Composition
Contents | Amount |
2×Mouse Tissue Direct PCR Mix | 5×1.0 mL |
Lysis Buffer | 2×20 mL |
Proteinase K | 800 μL |
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Q1: Why there is no ORF in the final result?
A1:
1) Excessive cleavage products. Select the most suitable template dosage, generally not more than 1/10 of the system;
2) The sampling volume is too large. Dilute the cleavage product by 10 times and then amplify, or reduce the sampling amount and re-lyse;
3) The tissue sample is not fresh. Fresh tissue samples are recommended;
4) Poor primer quality. Use genomic DNA for amplification to verify primer quality and optimize primer design.
Q2: Why does the final result show non-specific amplification?
A2:
1) The annealing temperature is too low and the number of cycles is too high. Increase the annealing temperature and reduce the number of cycles;
2) The template concentration is too high. Reduce the amount of template or dilute the template by 10 times for amplification;
3) Poor primer specificity. Optimize primer design.
Manual: Click to download
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