FlaPure Bacteria Genomic DNA Extraction Kit (DE703-50)

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FlaPure Bacteria Genomic DNA Extraction Kit (DE703-50)

This product is suitable for genomic DNA extraction from bacterial samples.

List price：＄ 450.00

Price：＄ 450.00

Amount：

50 rxns

Cat. No.：

DE703-50

Quantity：

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Product Description

This product is suitable for genomic DNA extraction from bacterial samples.

Highlights

1) High DNA quality: The extracted DNA has high concentration and purity, which is suitable for downstream experiments that require high concentration, purity and integrity.

2) Safe and low toxicity: No toxic reagents such as phenol and chloroform are required.

Introduction

This kit can rapidly extract high-quality genomic DNA from various bacteria (gram-negative and gram-positive bacteria), and can process 10⁶~10⁸ cells per time. The kit adopts an optimized buffer system, so that the DNA in the lysate can be efficiently and specifically bound to the silica matrix adsorption column. The extraction process does not require the use of toxic reagents such as phenol or chloroform, and the obtained DNA has high concentration and purity, and can be directly used in downstream experiments such as enzyme digestion, PCR, library construction, Southern Blot, and molecular labeling.

Composition

Contents	Amount(50 rxns)
Buffer GB1	15 mL
Buffer GB2	15 mL
Buffer GW1	13 mL
Buffer GW2	15 mL
Buffer TE	15 mL
Proteinase K(20 mg/mL)	1.0 mL
FlaPure DNA Columns	50
Collection Tubes(2.0 mL)	50

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Q1：Why are the columns clogged?

A1：

1) Too much bacterial solution or insufficient sample lysis. It is recommended to extract according to the recommended amount of the manual.

2) The centrifugation temperature is too low. The operation of this product is carried out at room temperature.

Q2：Why DNA yields are low?

A2：

1) The amount of bacterial liquid is too much or too little. It is recommended to extract according to the recommended amount of the manual.

2) The bacterial liquid is not fresh. It is recommended to extract fresh or high-viability bacterial liquid (such as log-phase bacteria).

Q3：Why Late Experiments Are Influenced by RNA?

A3：

1) RNase A digestion was not added. If the subsequent experiments need to remove the influence of RNA, RNase A can be added to digest according to the instructions.

2) Too much RNA in the sample. The amount of RNase A can be appropriately increased or the digestion time can be extended.

Q4：Why does the result show that there are more protein residues?

A4：

Too much bacterial fluid. It is recommended to reduce the amount of bacterial solution or repeat step 7 once.

Manual: Click to download

[1] Tang SM, Chen DC, Wang S, et al. Morphological and molecular analyses reveal two new species of Grifola (Polyporales) from Yunnan, China. MycoKeys. 2024,102:267-284.

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