Each bag of TBST instant granules can be formulated with 1 L 1× TBST buffer, primarily for washing after incubation of primary or secondary antibodies in Western Blot experiments, containing Tween-20.
Introduction
TBST is white to off-white instant granules, each bag of TBST instant granules can be prepared with 1L 1× TBST buffer solution, which is easy to operate and use. The main components of TBST are sodium chloride, potassium chloride, Tris buffered saline, and Tween-20. 1× TBST buffer contains sodium chloride at a concentration of 137 mM, potassium chloride at a concentration of 2.7 mM, and Tris buffered saline at a concentration of 137 mM. The concentration was 25 mM, and the content of Tween-20 was 0.05%. The pH value of the 1× solution is 7.4±0.15 (25℃).
TBST is a buffer widely used in biology, mainly used for immunohistochemistry, in situ hybridization, ELISA, preparation of blocking solution in western blotting experiments, preparation of primary or secondary antibodies, primary or secondary antibodies Anti-incubation washing, etc.