Introduction

      BCA protein quantification is currently the most widely used protein quantitation method. This product is an enhanced BCA protein quantification kit developed on the basis of BCA protein quantitative, which realizes the simplicity, high stability, high sensitivity and high compatibility of protein concentration determination. Compared to ordinary BCA protein quantification kits, the color development speed is faster, and the same sample can be incubated for a short time for absorbance determination.

      This product has a good linear relationship in the concentration range of 20~1000 μg/mL. The lower limit of detection concentration can reach 10 μg/mL, and the minimum detected protein amount can reach 0.2 μg. The volume of the sample to be tested is 1~20 μL.

      This product can be compatible with 5% SDS, 5% Triton X-100, 5% Tween 20(60, 80), affected by chelating agents and slightly higher concentrations of reducing agents, but it is necessary to ensure that there is no EGTA, EDTA is less than 10 mM, deoxidizer DTT is less than 1 mM, β-Mercaptoethanol less than 1 mM. If the sample diluent or lysate itself has a high background and the BCA method is not applicable, please use other methods to determine the protein concentration.

Composition

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