This product has a broad spectrum of protease inhibitor activity, but not phosphatase inhibitor activity, which can effectively inhibit the destruction of protease to the target protein in the process of protein purification, which is suitable for protein purification, protein immune imprinting, protein (total) precipitation, protein fluorescence and other experiments
Introduction
The crude protein extract after cell lysis contains a large number of endogenous proteases that degrade the proteins of cell extract. Therefore, in order to prevent the target protein from being destroyed by protease during protein purification, it is necessary to add protease inhibitors to inhibit its activity. There are many kinds of protease inhibitors, including EDTA, E-64, NaVO3, Bestatin, Leupetin, Pepstatin A, Aprotinin, etc. can effectively inhibit the degradation of proteins and maintain the interactions between proteins.
This product is a solution dissolved in DMSO, provided in the form of 100 × storage solution, mainly composed of Leiptetin, Pepstatin A, Aprotinin, E-64, etc., EDTA-free, does not have phosphatase inhibitory activity. The mixture can effectively inhibit the vast majority of protease activities, including serine protease, cysteine protease, pepsin, trypsin, papain, etc., suitable for extracting proteins from mammalian tissues and cells, and the resulting proteins can be used in Western Blot, Co-Immunoprecipitation, immunfluorescence and other experiments.
Composition
Contents | Amount |
Protease Inhibitor Cocktail，EDTA-free (100×) |
1.0 mL |
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