Introduction

    RIPA Lysis Buffer is a traditional rapid lysis buffer of cellular tissues that is primarily used for soluble proteins extracted from animal tissues and animal cells. There are many kinds of formulas, According to the strength of the lysis buffer, it can be roughly divided into three categories: enhanced, medium and weak.

    This product is a lysis buffer that uses classical tissue cells to rapidly lyse and obtain total protein, with a strong degree of lysis, and its lysis strength is greater than that of RIPA lysis buffer (medium) and NP-40 lysis buffer. The protein samples obtained by lysis can be used for conventional PAGE, Immunol Precipitation (IP), Western, etc. This product contains protease inhibitor ingredients that effectively inhibit protein degradation.

    Protein samples lyzed with RIPA lysis buffer can be using the BCA Protein Concentration Determination Kit. However, due to the higher concentration of detergent, the protein concentration of the sample obtained by the lysis of this lysate cannot be determined by Bradford method.

Composition

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