FlaPure Endotoxin-Free Maxi Plasmid Kit(Plus)(PE718-10)

English 简体中文

Welcome to Genesand Biotech Co.,Ltd

0086010-400-688-7890

PRODUCTS

HOME >> Nucleic Acid Isolation >> Plasmid DNA Extraction >> FlaPure Endotoxin-Free Maxi Plasmid Kit(Plus)(PE718-10)

FlaPure Endotoxin-Free Maxi Plasmid Kit(Plus)(PE718-10)

The kit is simple and time-saving, and the plasmid purity and yield are high; It is suitable for cell transfection and other routine plasmid experiments

List price：＄ 200.00

Price：＄ 200.00

Amount：

10 rxns

Cat. No.：

PE718-10

Quantity：

Buy now

Add to cart

Product Description

The kit is simple and time-saving, and the plasmid purity and yield are high; It is suitable for cell transfection and other routine plasmid experiments

Highlights

1) Easy to operate: endotoxin is quickly removed on the column, and other steps are also simple and efficient.

2) High purity: the precipitation is dense, and the impurities are removed cleanly.

3) Color indication: The key step color change indication judgment.

Introduction

This kit adopts an improved alkaline lysis treatment method and silica gel membrane adsorption technology, which can specifically and efficiently obtain high-purity plasmid DNA without endotoxin; endotoxin is directly removed on the column, which is easy to operate. The obtained plasmid can be directly used in molecular biology experiments such as cell transfection, enzyme digestion, ligation, transformation, PCR and sequencing.

For high-copy plasmids, 500~1500 ug plasmids can usually be obtained from 100 mL of bacterial solution, and 200~600 ug of plasmids can usually be obtained from 200 mL of bacterial solutions for low-copy plasmids. From the comprehensive evaluation of simplicity, yield and purity, this kit is superior to the common brands on the market.

Composition

Contents	Amount
RNase A (10 mg/mL)	1.1 mL
Buffer BL	30 mL
Buffer P1	110 mL
Buffer P2	110 mL
Buffer P4	110 mL
Endotoxin Removal Buffer	30 mL
Buffer W1	75 mL
Elution Buffer	25 mL
Adsorption Column EC (with Collection Tubes)	10
Collection Tubes	10

Related products

Type

Composition

Cat. No.

Amount

Introduction

Nucleic Acid

Electrophoresis

Agarose

AG801

100 g

High purity and clear background

One Step Rapid

Cloning

Uniclone One Step Seamless Cloning Kit

SC612

50 rxns

Different from the traditional seamless cloning kit, the kit can easily achieve 2~6 fragments seamless cloning.

Q1：Why is plasmid DNA yield low?

A1：

1) Plasmid copy number is low, plasmid >10 kb or gram-positive bacterial plasmid. The amount of bacteria used should be increased, 300~500 mL of overnight culture can be used, and the eluent Buffer EB should be preheated in a 60°C water bath, and the adsorption and elution time can be appropriately extended to increase the extraction efficiency;

2) The problem of bacteria species. There is a loss of plasmids during the storage process of the strains. Before culturing the bacteria, it is recommended to streak and activate to stabilize the yield;

3) The bacteria are not fully lysed. Bacteria must be fully resuspended in Buffer I/RNase A or the bacterial cells should not be too large to avoid clumping or excessive bacteria that cannot be lysed and reduce the yield.

Q2：Why is there genomic DNA contamination in plasmid DNA?

A2：

1) The bacterial culture time is too long. The culture time of bacterial liquid should be controlled within 12-16 h;

2) The cracking problem. When adding Buffer II, it must be mixed by gentle inversion; when processing multiple samples, the total time should not exceed 5 min from the time of adding Buffer II.

Manual: Click to download