There are DNase and RNase inhibitors in the master mix. Genome removal and reverse transcription can be completed in one step. The reaction temperature was 50°C.
1) Using antibody-modified new hot-start GS AntiQ DNA polymerase and carefully optimized Buffer;
2) This product contains a unique calibration dye that is compatible with a range of qPCR instruments, including those that require ROX calibration, and there is no need to add additional dyes to calibrate the instrument during the experiment.