To help life science research,
let more research results benefit mankind
To help life science research,
let more research results benefit mankind
This product is suitable for conventional PCR and hot-start PCR.
Highlights
Simple operation: 2×Mix, only need to add primers, template and ddH2O for PCR amplification.
Instruction
This product contains antibody-modified hot-start GS Antitaq DNA polymerase, dNTPs and an optimized buffer system at a concentration of 2× PCR amplification can be performed only by adding primers, templates and ddH2O.
The PCR product amplified by this product has a protruding "A" base at the 3' end, which can be directly used for TA cloning after purification.
Composition
Contents | Amount |
2×GS Antitaq PCR Mix | 5×1.0 mL |
Related products
Type | Composition | Cat. No. | Amount | Introduction |
High-Fidelity PCR | SF001 | 5×1.0 mL | The fidelity is 60 times that of wild-type Taq enzyme; Extremely fast extension speed: 10~30 s/kb. | |
Nucleic Acid Electrophoresis | AG801 | 100 g | High purity and clear background | |
Topo Cloning | TC601 | 20 rxns | This product is suitable for TA cloning of DNA fragments within 5 kb; The connection reaction is completed within 5 min; Not contain polyclonal restriction sites. |
Q1:Why is there no product or a small amount of product in the final result?
A1:
1)Template degradation or poor template purity. Use electrophoresis to check template quality and use high-quality templates.
2)The template concentration is too low. Appropriately increase the template usage.
3)The primers are not suitable. Optimize primer design.
4)The annealing temperature is not suitable. Set the annealing temperature gradient and explore the appropriate annealing temperature.
5)The number of cycles is too small. Appropriately increase 5~10 cycles.
6)Insufficient extension time. For complex templates, the extension speed can be appropriately increased to 20~30 s/kb.
Q2:Why do non-specific bands or diffuse bands appear in amplification?
A2:
1)Poor primer specificity. Optimize primers to avoid nonspecific bands and amplification of primer dimers.
2)The primer concentration is too high. Decrease primer concentration.
3)Template excess or poor purity. Reduce the amount of templates and use high-quality templates.
4)The annealing temperature is too low. Appropriately increase the annealing temperature or use the Touchdown PCR program.
5)Too many cycles. Reduce the number of cycles to 25~30 cycles.
Manual: Click to download
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