Highlights

1. Quick connection, the fastest only 10 min.

2. High purity, no nuclease activity residue.

Introduction

        T4 DNA Ligase is expressed from E. coli carrying the T4 DNA ligase gene. The enzyme can catalyze the formation of phosphodiester bonds between 5'-phosphate groups and 3'-hydroxyl groups between double helix DNA or RNA. The enzyme can repair single-stranded nicks between double-stranded DNA, RNA or DNA/RNA complexes, and can ligate DNA fragments with cohesive or blunt ends, but is inactive for single-stranded nucleic acids. T4 DNA Ligase requires ATP as a cofactor and completes the cohesive end ligation reaction in as little as 10 minutes at room temperature.

Composition

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