GS-GelRed (10,000×) (GL802)

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GS-GelRed (10,000×) (GL802)

1)Safe and non-toxic.
2)High sensitivity.

List price：＄ 600.00

Price：＄ 600.00

Cat. No.：

GL802

Amount：

500 μL

Quantity：

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Product Description

1)Safe and non-toxic.
2)High sensitivity.

Product Highlights

1) Safe and non-toxic: GS-GelRed's unique oiliness and high molecular weight make it unable to penetrate the cell membrane and enter the cell.

2) High sensitivity: It is suitable for DNA electrophoresis of various molecular weights, and has higher detection sensitivity for trace amounts of DNA.

3) High signal-to-noise ratio: strong sample fluorescence signal and low background signal.

4) Wide range of applications: both gel staining (staining before electrophoresis) and bubble staining (staining after electrophoresis); suitable for agarose gel or polyacrylamide gel electrophoresis; can be used for dsDNA, ssDNA and RNA staining.

Introduction

GS-GelRed is a safe, sensitive and stable fluorescent nucleic acid gel staining reagent, suitable for dsDNA, ssDNA and RNA staining on agarose or polyacrylamide gels. The DNA bands stained by this product are projected by UV light. It exhibits red fluorescence and can replace ethidium bromide (EtBr). Ames' test results also show that the dye is far less mutagenic than ethidium bromide (EtBr), making it safer to use.

GS-GelRed and EB have the same spectral characteristics, no need to replace the imaging system, just use the same common UV gel transilluminator to observe EB, and the best excitation can be obtained near 300 nm UV light. Note that GS-GelRed cannot be fully excited by a 488 nm argon-ion laser (such as a blue transilluminator) or visible light of similar wavelengths, so imaging systems using such excitation devices are not recommended.

Composition

Contents

Amount

GS-GelRed(10,000×)

500 μL

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Q1：Why are DNA results scattered, tailed or bent when using gel staining?

A1：

1) Make sure the final concentration of the dye used is 1×.

2) The amount of DNA loaded is too large. Reduce DNA sample loading to 1/3 or 1/5.

3) The gel concentration is not suitable. It is recommended to use a low-concentration agarose gel for the detection of large fragments of DNA.

4) Excessive loading buffer in the sample. SDS in the loading buffer may affect the electrophoresis effect, it is recommended to reduce the amount of loading buffer.

5) Use a suitable voltage, 5~10 V/cm is recommended.

6) Use fresh running buffer.

Q2：Why Differences in DNA Mobility Are Found When Using Gel Staining?

A2：

1) Reduce the amount of DNA sample loading to 1/3 or 1/5;

2) The molecular weight of GS-GelRed is large, and the migration rate of DNA due to large molecular weight may be affected by the ratio of dye to DNA. Therefore, it is not recommended to add GS-GelRed directly to the loading buffer, which will lead to inaccurate band migration;

3) The size of DNA bands can be accurately determined by bubble staining.

Q3：Why ssDNA and RNA do not stain as well as dsDNA?

A3：

The sensitivity of GS-GelRed to dsDNA is 5 times that of ssDNA or RNA, and the amount of ssDNA or RNA can be appropriately increased during the operation.

Manual: Click to download

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