FlaPure Gel Purification Kit (GE706)

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let more research results benefit mankind

To help life science research,

let more research results benefit mankind

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FlaPure Gel Purification Kit (GE706)

One box of dual-purpose, can effectively recover 80 bp ~10 kb double-stranded DNA fragments, can also directly recover PCR stock solution, gel without weighing, easy to operate, recovery rate of up to 85%.

List price：＄ 380.00

Price：＄ 380.00

Cat. No.：

GE706

Amount：

200 rxns

Quantity：

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Product Description

One box of dual-purpose, can effectively recover 80 bp ~10 kb double-stranded DNA fragments, can also directly recover PCR stock solution, gel without weighing, easy to operate, recovery rate of up to 85%.

Highlights

1) Fast: the glue block does not need to be weighed, and the operation is simple.

2) High efficiency: high recovery efficiency and high purity of the obtained target DNA.

3) Wide applicability: one box can be used for both gel recovery of DNA fragments and direct recovery of PCR products.

Introduction

This kit is suitable for recovering up to 10 μg DNA (80 bp~10 kb) from agarose gel, and the recovery rate can reach 65~85%. After the agarose gel was dissolved in high-order salt (Buffer GN), the DNA fragments were selectively adsorbed on the silica matrix membrane in the spin column. The recovered DNA has high purity and can maintain fragment integrity and high biological activity, and can be directly used in molecular biology experiments such as sequencing, ligation, PCR amplification, and in vitro transcription.

Composition

Contents	Amount(200 rxns)
Buffer BL	55 mL
Buffer GN	110 mL
Buffer W1	2×72 mL
Elution Buffer	25 mL
EC	200

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One Step Rapid Cloning	Uniclone One Step Seamless Cloning Kit	SC612	50 rxns	Different from the traditional seamless cloning kit, the kit can easily achieve 2~6 fragments seamless cloning.

Q1：Why is the DNA recovery rate low in the end result?

A1：

1) The agarose gel is not completely melted. Cut off the agarose that does not contain the target fragment as much as possible, and invert the gel upside down several times to fully melt the gel to ensure that no solid agarose remains.

2) The reagent preparation is incorrect. Buffer W1 needs to add the specified volume of absolute ethanol according to the label on the bottle.

3) The elution efficiency is low. Preheat the Elution Buffer to 60~65℃, and perform the second elution.

Q2：Why Purified DNA Downstream Results are unsatisfactory?

A2：

1) Residual salt ions. Make sure to elute twice with Buffer W1. In addition, add Buffer W1 around the wall of the adsorption column, or add Buffer W1 and then invert and mix for 2 to 3 times to completely flush out the salt ions adhering to the tube wall.

2) Agarose residue. Cut off the agarose that does not contain the target fragment as much as possible, and invert it upside down several times to fully melt the gel to ensure that no solid agarose remains.

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