Genesand dNTP Mix contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 25 mM. The nucleotides have greater than 99% purity, are free of nuclease activities, human and E. coli DNA. Mixes offer the possibility to reduce the number of pipetting steps and the risk of reaction set up errors. They are designed for many different molecular biology applications.
There are DNase and RNase inhibitors in the master mix. Genome removal and reverse transcription can be completed in one step. The reaction temperature was 50°C.
1) Using antibody-modified new hot-start GS AntiQ DNA polymerase and carefully optimized Buffer;
2) This product contains a unique calibration dye that is compatible with a range of qPCR instruments, including those that require ROX calibration, and there is no need to add additional dyes to calibrate the instrument during the experiment.