FlaPure Animal Tissue/Cell /Blood DNA Extraction Kit (DE712-50)

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FlaPure Animal Tissue/Cell /Blood DNA Extraction Kit (DE712-50)

This kit can rapidly extract genomic DNA from ≤ 25 mg fresh or frozen animal tissues (such as rat tail, rat toe, liver, etc.), blood, and cells.

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Cat. No.：

DE712-50

Amount：

50 rxns

Quantity：

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Product Description

This kit can rapidly extract genomic DNA from ≤ 25 mg fresh or frozen animal tissues (such as rat tail, rat toe, liver, etc.), blood, and cells.

Introduction

This kit can rapidly extract genomic DNA from ≤ 25 mg fresh or frozen animal tissues (such as rat tail, rat toe, liver, etc.), blood, and cells. The kit adopts an optimized buffer system, so that the DNA in the lysate can be efficiently and specifically bound to the silica matrix adsorption column. The extraction process does not require the use of toxic solvents such as phenol or chloroform, and the obtained DNA has high concentration and purity, and can be directly used in downstream experiments such as enzyme digestion, PCR, library construction, Southern Blot, and molecular labeling.

Composition

Contents	Amount(50 rxns)
Buffer GA1	15 mL
Buffer GA2	15 mL
Buffer GW1	13 mL
Buffer GW2	15 mL
Buffer TE	15 mL
Proteinase K(20 mg/mL)	1.0 mL
FlaPure DNA Columns	50
Collection Tubes(2.0 mL)	50

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Q1: Why are the columns clogged?

A1:

1) Too much sample is used. Especially for DNA-rich tissues, it is necessary to reduce the amount.

2) The sample was not sufficiently lysed or ground and overnight digestion is recommended for rat tail tissue.

Q2: Why DNA yields are low?

A2:

1) Insufficient sample lysis process. If the sample is excessive, reduce the amount of sample appropriately, extend the incubation time at 56˚C, or add 20 μL Proteinase K (20 mg/mL) to digest in step 2.

2) The amount of sample is too small.

3) The quality of the sample material is not good.

Q3: Why late-stage experiments are affected by RNA?

A3: No RNase A digestion was added or the RNA content in the sample is too high(the amount of RNase A can be appropriately increased or the digestion time can be extended).